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publication info
R. Simpson, Cold Spring Harbor Laboratory Press, 2003, 926 pp., hard cover
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available only in EU
not available in ROW, USA and Canada
General description
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide–ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.
Table of Contents
Chapter 1. Introduction to Proteomics
Chapter 2. One–dimensional Polyacrylamide Gel Electrophoresis
Chapter 3. Preparing Cellular and Subcellular Extracts
Chapter 4. Preparative Two–dimensional Gel Electrophoresis with Immobilized pH Gradients
Chapter 5. Reversed–phase High–performance Liquid Chromatography
Chapter 6. Amino– and Carboxy– terminal Sequence Analysis
Chapter 7. Peptide Mapping and Sequence Analysis of Gel–resolved Proteins
Chapter 8. The Use of Mass Spectrometry in Proteomics
Chapter 9. Proteomic Methods for Phosphorylation Site Mapping
Chapter 10. Characterization of Protein Complexes
Chapter 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein′s Structure, Functions, and Interactions
Chapter 2. One–dimensional Polyacrylamide Gel Electrophoresis
Chapter 3. Preparing Cellular and Subcellular Extracts
Chapter 4. Preparative Two–dimensional Gel Electrophoresis with Immobilized pH Gradients
Chapter 5. Reversed–phase High–performance Liquid Chromatography
Chapter 6. Amino– and Carboxy– terminal Sequence Analysis
Chapter 7. Peptide Mapping and Sequence Analysis of Gel–resolved Proteins
Chapter 8. The Use of Mass Spectrometry in Proteomics
Chapter 9. Proteomic Methods for Phosphorylation Site Mapping
Chapter 10. Characterization of Protein Complexes
Chapter 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein′s Structure, Functions, and Interactions
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