Z701483
Protein Purification Protocols, 2nd ed.
experts update this classic protocols manual for protein purification techniques
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
ISBN-10:
1-58829-067-0
ISBN-13:
978-1-58829-067-0
UNSPSC Code:
55101509
Recommended Products
publication info
P. Cutler, ed., Humana Press, 2003, 512 pp., hard cover
manufacturer/tradename
Humana
General description
Experts update this classic protocols manual for protein purification techniques. New chapters include those on protein fractionation and chromatographic techniques. New developments in proteomics are included such as the use of 2-D gel electrophoresis as a preparative technique for protein characterization.
They also include newer techniques using analytical chromatography for the multidimensional separations of proteins and peptides as well as mass spectrometric techniques for isolating proteins. The authors provide descriptions of how to scale-up purification methods and the purification of proteins for therapeutic use.
They also include newer techniques using analytical chromatography for the multidimensional separations of proteins and peptides as well as mass spectrometric techniques for isolating proteins. The authors provide descriptions of how to scale-up purification methods and the purification of proteins for therapeutic use.
Table of Contents
General Strategies
Preparation of Extracts from Animal Tissues
Protein Extraction from Plant Tissues
Extraction of Recombinant Protein from Bacteria
Protein Extraction from Fungi
Preparation of Extracts from Animal Tissues
Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria
The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds
Avoidance of Proteolysis in Extracts
Concentration of Extracts
Making and Changing Buffers
Purification and Concentration by Ultrafiltration
Bulk Purification by Fractional Precipitation
Ion Exchange Chromatography
Hydrophobic Interaction Chromatography
Affinity Chromatography
Dye-Ligand Affinity Chromatography
Lectin Affinity Chromatography
Immunoaffinity Chromatography
Immobilized Metal Ion Affinity Chromatography
Chromatography on Hydroxyapatite
Thiophilic Affinity Chromatography and Related Methods
Affinity Precipitation Methods
Isoelectric Focusing
Chromatofocussing
Size-Exclusion Chromatography
Fast Protein Liquid Chromatography
Reversed-Phase Chromatography of Proteins
Extraction of Membrane Proteins
Removal of Detergent from Protein Fractions
Purification of Membrane Proteins
Lyophilization of Proteins
Storage of Pure Proteins
Electroelution of Proteins from Polyacrylamide Gels
Electroblotting of Proteins from Polyacrylamide Gels
Two Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses
Microscale Solution Isoelectrofocusing: A Sample Prefractionation
Method for Comprehensive Proteome Analysis
Practical Column Chromatography
Detection Methods
Peptide Proteomics: Quantitative Profiling of Endogenous Low Molecular Weight Polypeptides by Multidimensional Chromatographic Analysis
Multidimensional Liquid Chromatography of Proteins
Mass Spectrometry
Purification of Therapeutic Proteins
Purification Process Scale-Up
Preparation of Extracts from Animal Tissues
Protein Extraction from Plant Tissues
Extraction of Recombinant Protein from Bacteria
Protein Extraction from Fungi
Preparation of Extracts from Animal Tissues
Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria
The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds
Avoidance of Proteolysis in Extracts
Concentration of Extracts
Making and Changing Buffers
Purification and Concentration by Ultrafiltration
Bulk Purification by Fractional Precipitation
Ion Exchange Chromatography
Hydrophobic Interaction Chromatography
Affinity Chromatography
Dye-Ligand Affinity Chromatography
Lectin Affinity Chromatography
Immunoaffinity Chromatography
Immobilized Metal Ion Affinity Chromatography
Chromatography on Hydroxyapatite
Thiophilic Affinity Chromatography and Related Methods
Affinity Precipitation Methods
Isoelectric Focusing
Chromatofocussing
Size-Exclusion Chromatography
Fast Protein Liquid Chromatography
Reversed-Phase Chromatography of Proteins
Extraction of Membrane Proteins
Removal of Detergent from Protein Fractions
Purification of Membrane Proteins
Lyophilization of Proteins
Storage of Pure Proteins
Electroelution of Proteins from Polyacrylamide Gels
Electroblotting of Proteins from Polyacrylamide Gels
Two Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses
Microscale Solution Isoelectrofocusing: A Sample Prefractionation
Method for Comprehensive Proteome Analysis
Practical Column Chromatography
Detection Methods
Peptide Proteomics: Quantitative Profiling of Endogenous Low Molecular Weight Polypeptides by Multidimensional Chromatographic Analysis
Multidimensional Liquid Chromatography of Proteins
Mass Spectrometry
Purification of Therapeutic Proteins
Purification Process Scale-Up
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
