T1827

Anti -TBP antibody, Mouse monoclonal

clone 58C9, purified from hybridoma cell culture

• Synonym(s): Anti-GTF2D, Anti-GTF2DI, Anti-SCA17, Anti-TATA box binding protein, Anti-TFIID
• MDL number: MFCD01322691
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 58C9
• mol wt: antigen ~42 kDa
• species reactivity: Sf9 cell line, human, yeast, Drosophila melanogaster
• technique(s): immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells
• isotype: IgG2b
• UniProt accession no.: P20226
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Drosophila melanogaster ... Tbp(37476) ; human ... TBP(6908)

Description

General description

Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs).

TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells.

Immunogen

Drosophila TFIID complex.

Application

Monoclonal Anti-TBP antibody produced in mouse has been used in:

• immunoblotting
• co-immunoprecipitation
• immunohistochemistry

Biochem/physiol Actions

TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters.

The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17).

Target description

TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)