SAB4200509

Anti-ARG1 antibody, Mouse monoclonal

clone ARG1-6, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-ARG1 antibody produced in mouse, AntiArginase 1, AntiArginase, liver, AntiLiver-type arginase, AntiOTTHUMP00000017209, AntiType I arginase
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ARG1-6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~40 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5.0 μg using lysates of HEK-293T cells overexpressing human ARG1.; western blot: 0.25-0.50 μg/mL using whole extracts of HEK-293T cells overexpressing human ARG1.
• UniProt accession no.: P05089
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ARG1(383)

Description

General description

Monoclonal Anti-ARG1 (mouse IgG2b isotype) is derived from the hybridoma ARG1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The type I isoform encoded by the ARG1 gene is a cytosolic enzyme expressed predominantly in the liver as a component of the urea cycle. Arginase 1 (ARG1) gene is mapped on the human chromosome at 6q23.2. Two transcript variants encoding different isoforms have been found for this gene.

Specificity

Monoclonal Anti- ARG1, clone ARG1-6 recognizes human ARG1.

Immunogen

synthetic peptide corresponding to an internal region of human ARG1, conjugated to KLH. The corresponding sequence differs by 3 amino acids in rat and by 4 amino acids in mouse ARG1.

Application

Monoclonal Anti-ATG101 antibody produced in mouse is suitable for use in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Arginase 1 (ARG1) plays a role in removing nitrogen by catalyzing the hydrolysis of arginine to urea and ornithine. Deficiency of this enzyme is associated with argininemia, an inherited autosomal recessive disorder characterized by hyperammonemia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable