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MAK187

Sigma-Aldrich

Total Antioxidant Capacity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

TAC Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases; rheumatological diseases

storage temp.

2-8°C

General description

Oxidants, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS), can generate free radicals that can cause severe oxidative damage to cellular lipids, membranes, proteins, and DNA.[1][2] Antioxidants can scavenge these free radicals and prevent cellular oxidative stress by enzymatic andnon-enzymatic mechanisms. Enzyme systems that function as antioxidants include catalase and peroxidase. Tocopherols, carotenes, vitamin A, and ubiquinols function as lipid-soluble antioxidants; whereas, glutathione and ascorbate are some of the water-soluble antioxidants.[3] Measurement of the total non-enzymatic antioxidant capacity (TAC) of biological samples is indicative of their ability to counteract oxidative stress-induced damage in cells. TAC is used to provide insights into the development and treatment of oxidative-stress related disorders.[4]

In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Cu2+ ion is converted to Cu+ by both small molecules and proteins. However, the use of the Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion chelates with a colorimetric probe, giving a broad absorbance peak at ~570 nm, which is proportional to the total antioxidant capacity. The kit gives antioxidant capacity in Trolox equivalents (ranging from 4-20 nmole/well). Trolox, a water-soluble vitamin E analog, serves as an antioxidant standard.

application

Total antioxidant capacity assay kit has been used to determine total antioxidant capacity of leaf tissue samples.[5][6]

Suitability

This kit is suitable for the detection of small molecule and protein antioxidants in cell and tissue lysates, media, or in biological fluids such as urine, plasma, and serum.

Principle

In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Cu2+ ion is converted to Cu+ by both small molecules and proteins. However, the use of the Protein Mask prevents Cu2+ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion chelates with a colorimetric probe, giving a broad absorbance peak at ~570 nm, which is proportional to the total antioxidant capacity. The kit gives antioxidant capacity in Trolox equivalents (ranging from 4-20 nmole/well). Trolox, a water-soluble vitamin E analog, serves as an antioxidant standard.

Kit Components Only

Product No.
Description
  • Cu2+ Reagent
  • Assay Diluent
  • Protein Mask
  • Trolox Standard, 1 μmole

replaced by

Product No.
Description
Pricing

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

hcodes

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8K31K02740
8K20K02740
8F28K02740
8A21K02740
8B04K02740

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Niclas U Lundsgaard et al.
Conservation physiology, 8(1), coaa002-coaa002 (2020-05-30)
Multiple environmental changes are thought to be contributing to the widespread decline of amphibians in montane regions, but interactions between drivers of decline are not well understood. It has been proposed previously that elevated ultraviolet-B radiation (UBVR) and low temperatures
Harjeevan Kaur et al.
Plant biotechnology journal, 18(4), 983-991 (2019-09-26)
Oilseed rape (Brassica napus) is the third largest source of vegetable oil globally. In addition to food uses, there are industrial applications that exploit the ability of the species to accumulate the very-long-chain fatty acid (VLCFA) erucic acid in its
Free radicals: properties, sources, targets, and their implication in various diseases.
Phaniendra A, et al.
Indian Journal of Clinical Biochemistry : IJCB, 30(1), 11-26 (2015)
Simon Vitt et al.
The Science of the total environment, 712, 136341-136341 (2020-01-14)
Increasing UVB radiation (UVB) reaching earth's surface following stratospheric ozone depletion is linked to serious consequences for organisms. While studies have focused on direct cytocidal and immunomodulatory effects of UVB, indirect consequences for fitness-related life-history traits are largely unexplored, although
UV-B antagonises shade avoidance and increases levels of the flavonoid quercetin in coriander (Coriandrum sativum).
Fraser D P, et al.
Scientific Reports, 7(1), 17758-17758 (2017)
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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the lower detection limit?

    1 answer

    1. Samples should have an absorbance within the linear range of the standard curve, which is 0 – 20 nmol/well. If the absorbance falls outside this range, it is recommended to redilute and rerun the samples. The detection limit of the assay is approximately 0.1 nmol per well (or 1 µM) of Trolox.

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  4. Can the total antioxidative capacity kit measure cells treated with strongly colored dyes like methylene blue and detect absorbance differences at 685nm?

    1 answer

    1. A 100 nm difference in wavelength for peak absorbance suggests that methylene blue should not interfere with the assay. Theoretically, the kit should work, but it's advisable for the customer to conduct their own tests to determine if sample dilutions are necessary.

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