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H3506

Sigma-Aldrich

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
Enzyme Commission number:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type I-S

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt accession no.

Q5E985
Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus.
Kirshenbaum LA et al.
The Journal of Clinical Investigation, 92, 381-381 (1993)
Heikki J Nieminen et al.
Scientific reports, 7(1), 3991-3991 (2017-06-24)
Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound
Moshe Sade-Feldman et al.
Nature communications, 8(1), 1136-1136 (2017-10-27)
Treatment with immune checkpoint blockade (CPB) therapies often leads to prolonged responses in patients with metastatic melanoma, but the common mechanisms of primary and acquired resistance to these agents remain incompletely characterized and have yet to be validated in large
A SAGE (serial analysis of gene expression) view of breast tumor progression.
Porter DA et al.
Cancer Research, 61, 5697-5697 (2001)
Haoheng Yan et al.
The American journal of pathology, 176(3), 1421-1432 (2010-01-30)
Mutation and loss of function in p53 are common features among human breast cancers. Here we use BALB/c-Trp53+/- mice as a model to examine the sequence of events leading to mammary tumors. Mammary gland proliferation rates were similar in both
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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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