F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

• Synonym(s): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• product name: Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture
• biological source: human foreskin fibroblasts
• Quality Level: 200
• product line: BioReagent
• form: lyophilized powder
• mol wt: ~550 kDa
• packaging: pkg of 0.5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑5 μg/cm²
• impurities: Human Source, none detected (HCV); Human Source, none detected (HIV); Human Source, none detected (HTLV); Human Source, none detected (HbSAg); Microbial Contamination, passes test
• solubility: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• NCBI accession no.: NM_212482.4
• UniProt accession no.: P02751
• binding specificity: Peptide Source: Collagen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... FN1(2335)

Description

General description

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

Application

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

Biochem/physiol Actions

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

Features and Benefits

• Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
• It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
• The starting material is tested and found negative for HIV and HBsAg.
• The product is soluble in sterile water, yielding a clear, colorless solution.
• It can be stored at -20°C for at least three years.

Caution

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

Physical form

Lyophilized in CAPS buffered saline

Preparation Note

dialyzed against chloroform

Reconstitution

Store reconstituted solution in working aliquots at -20°C or lower.

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)