M8194
Anti-MAFF (C-Terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-v-maf Avian Musculoaponeurotic Fibrosarcoma Oncogene Family, ProteinF
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About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 18 kDa
species reactivity
human
technique(s)
immunoprecipitation (IP): 2-4 μL using A431 cell line
microarray: suitable
western blot: 1:2,000-1:4,000 using whole extracts of the A431 cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAFF(23764)
General description
The MAFF gene is located on the human chromosome at 22q13.1. The encoded protein is a small MAF protein and is expressed in the nucleus.
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) is a transcription factor which does not have a transactivation domain but possesses a leucine-zipper.
Specificity
Anti-MAFF (C-terminal) specifically recognizes MAFF.
Immunogen
synthetic peptide corresponding to amino acids 147-163 of human MAFF (isoform a). The immunizing peptide does not cross-react with other members of the family.
Application
Anti-MAFF (c-terminal) antibody produced in rabbit has been used in:
- western blotting
- chromatin immunoprecipitation (chip)
- co-immunoprecipitation
- immunoprecipitation
Biochem/physiol Actions
The small MAF proteins are associated with many physiological processes including development, differentiation, hematopoiesis and stress response. Interestingly, these proteins regulate the stress response via different mechanisms. The MAFF gene is down-regulated in diabetes mellitus 1 patient. This gene is underexpressed in acute lymphoblastic lymphoma (ALL) patients.
v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F (MAFF) has been shown to interact with other transcription factors and it associates with certain regulatory sequences in the gene encoding the oxytocin receptor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Meenakshi B Kannan et al.
Biochimica et biophysica acta, 1823(10), 1841-1846 (2012-06-23)
The small MAFs, MAFF, MAFG and MAFK have emerged as crucial regulators of mammalian gene expression. Previous studies have linked small MAF function, by virtue of their heterodimerization with the Cap 'n' Collar (CNC) family of transcription factors, to the
Zhenyi Qiao et al.
Frontiers in nutrition, 9, 799053-799053 (2022-04-05)
Metabolites of probiotics that are beneficial to human health have been isolated from the intestinal tract and natural dairy products. However, many studies on probiotics and prebiotics are limited to the observation of human cohorts and animal phenotypes. The molecular
Julie A Moran et al.
The Biochemical journal, 361(Pt 2), 371-377 (2002-01-05)
The three small Maf proteins, MafF, MafG and MafK, have been implicated in a number of physiological processes, including development, differentiation, haematopoiesis and stress response. Here we report the constitutive expression of mafF, mafG and mafK in six human cell
Eui Jung Moon et al.
Nature communications, 12(1), 4308-4308 (2021-07-16)
Hypoxia plays a critical role in tumor progression including invasion and metastasis. To determine critical genes regulated by hypoxia that promote invasion and metastasis, we screen fifty hypoxia inducible genes for their effects on invasion. In this study, we identify
Yaeko Takagi et al.
Biochemical and biophysical research communications, 320(1), 62-69 (2004-06-23)
Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. To date, three small Maf proteins, MafF, MafG, and MafK, have been reported in vertebrates, which share redundant functions. In this study, we tried to
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