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I6634

Sigma-Aldrich

Insulin from bovine pancreas

powder, BioReagent, suitable for cell culture

Synonym(s):

Bovine insulin, Cell culture grade insulin

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50 MG
$297.00
100 MG
$508.00
250 MG
$896.00
500 MG
$1,580.00
1 G
$2,730.00

$297.00

Estimated to ship onApril 19, 2025

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All Photos(5)

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50 MG
$297.00
100 MG
$508.00
250 MG
$896.00
500 MG
$1,580.00
1 G
$2,730.00

About This Item

Empirical Formula (Hill Notation):
C254H377N65O75S6
CAS Number:
11070-73-8
Molecular Weight:
5733.49
EC Number:
234-291-2
MDL number:
MFCD00131380
UNSPSC Code:
12352209
NACRES:
NA.77
biological source:
bovine
form:
powder

$297.00

Estimated to ship onApril 19, 2025

Request a Bulk Order

biological source

bovine

Quality Level

200

product line

BioReagent

form

powder

potency

≥25 USP units per mg

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

solubility

acetic acid: 2 mg/mL (pH: 2-3)
hydrochloric acid: 2 mg/mL (pH: 2-3)

UniProt accession no.

P01317

shipped in

ambient

storage temp.

−20°C

InChI key

IXIBAKNTJSCKJM-BUBXBXGNSA-N

Gene Information

cow ... INS(280829)

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Application

Insulin is used as a growth factor in many mammalian cell culture systems.
  • Insulin from bovine pancreas has been used for the differentiation of MSCs (mesenchymal stem cells) to adipocytes.[1]
  • It has been used for culturing of various cells.[2][3][4]
  • It has also been used to study insulin-mediated inhibition of the HMGCS2 (3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase) gene.[5]

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Other Notes

InChI: 1S/C254H377N65O75S6/c1-29-131(24)205(312-192(334)103-256)250(389)317-204(130(22)23)246(385)287-158(74-81-199(344)345)216(355)281-155(70-77-188(260)330)220(359)307-183-116-399-400-117-184-242(381)305-177(110-321)238(377)293-161(87-122(6)7)223(362)294-167(94-139-52-60-145(324)61-53-139)226(365)282-153(68-75-186(258)328)217(356)289-160(86-121(4)5)221(360)284-157(73-80-198(342)343)219(358)301-173(100-189(261)331)233(372)297-169(96-141-56-64-147(326)65-57-141)229(368)308-182(241(380)303-175(253(393)394)102-191(263)333)115-398-396-113-180(212(351)270-106-193(335)277-152(71-78-196(338)339)215(354)280-150(50-41-83-268-254(264)265)210(349)269-107-194(336)278-165(92-137-45-35-31-36-46-137)225(364)296-166(93-138-47-37-32-38-48-138)228(367)298-170(97-142-58-66-148(327)67-59-142)236(375)318-206(135(28)323)251(390)319-84-42-51-185(319)244(383)285-151(49-39-40-82-255)213(352)276-134(27)252(391)392)310-248(387)202(128(18)19)315-234(373)163(89-124(10)11)291-227(366)168(95-140-54-62-146(325)63-55-140)295-222(361)159(85-120(2)3)288-207(346)132(25)274-214(353)156(72-79-197(340)341)286-245(384)201(127(16)17)314-235(374)164(90-125(12)13)292-231(370)172(99-144-105-267-119-273-144)300-237(376)176(109-320)279-195(337)108-271-211(350)179(112-395-397-114-181(309-243(183)382)240(379)275-133(26)208(347)304-178(111-322)239(378)316-203(129(20)21)249(388)311-184)306-224(363)162(88-123(8)9)290-230(369)171(98-143-104-266-118-272-143)299-218(357)154(69-76-187(259)329)283-232(371)174(101-190(262)332)302-247(386)200(126(14)15)313-209(348)149(257)91-136-43-33-30-34-44-136/h30-38,43-48,52-67,104-105,118-135,149-185,200-206,320-327H,29,39-42,49-51,68-103,106-117,255-257H2,1-28H3,(H2,258,328)(H2,259,329)(H2,260,330)(H2,261,331)(H2,262,332)(H2,263,333)(H,266,272)(H,267,273)(H,269,349)(H,270,351)(H,271,350)(H,274,353)(H,275,379)(H,276,352)(H,277,335)(H,278,336)(H,279,337)(H,280,354)(H,281,355)(H,282,365)(H,283,371)(H,284,360)(H,285,383)(H,286,384)(H,287,385)(H,288,346)(H,289,356)(H,290,369)(H,291,366)(H,292,370)(H,293,377)(H,294,362)(H,295,361)(H,296,364)(H,297,372)(H,298,367)(H,299,357)(H,300,376)(H,301,358)(H,302,386)(H,303,380)(H,304,347)(H,305,381)(H,306,363)(H,307,359)(H,308,368)(H,309,382)(H,310,387)(H,311,388)(H,312,334)(H,313,348)(H,314,374)(H,315,373)(H,316,378)(H,317,389)(H,318,375)(H,338,339)(H,340,341)(H,342,343)(H,344,345)(H,391,392)(H,393,394)(H4,264,265,268)/t131-,132-,133-,134-,135+,149-,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,200-,201-,202-,203-,204-,205-,206-/m0/s1

Physical form

powder-0 °C; solution stable 2-8 °C, 1 year

Reconstitution

Insulin has low solubility at neutral pH. It can be solubilized at 2 mg/ml in dilute acetic or hydrochloric acid, pH 2-3.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000411957
0000411957
0000328086
0000328086
0000324253

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Boah Lee et al.
PloS one, 12(8), e0183569-e0183569 (2017-08-29)
Pancreatic islets can adapt to oscillatory glucose to produce synchronous insulin pulses. Can islets adapt to other oscillatory stimuli, specifically insulin? To answer this question, we stimulated islets with pulses of exogenous insulin and measured their Ca2+ oscillations. We observed
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1.
Nadal A et al.
The Biochemical Journal, 366, 289-289 (2002)
Major histocompatibility complex (MHC) class I gene expression in single neurons of the central nervous system: differential regulation by interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.
Neumann H et al.
The Journal of Experimental Medicine, 185, 305-305 (1997)
Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cord and mobilized adult blood are not.
Wexler SA et al.
British Journal of Haematology, 121, 368-368 (2003)
Lei Zhang et al.
Oncotarget, 7(49), 80382-80390 (2016-11-03)
Pleiotrophin (PTN) augments tumor growth by increasing proliferation of tumor cells and promoting vascular abnormalization, but its role in early gliomagenesis has not been evaluated. Through analysis of publically available datasets, we demonstrate that increased PTN mRNA expression is associated
View All Related Papers

Questions

1–9 of 9 Questions  

  1. How is your cell culture grade insulin from bovine pancreas product is extracted and purified? Is the pancreas tissue homogenized prior to insulin protein extraction? Are any steps taken to remove contaminants? What is the estimated purity?

    1 answer

    1. The manufacturing method is considered confidential. This product is not tested the purity. The potency is tested for each lot and reported on the Certificate of Analysis.

      Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/i6634#product-documentation

      Helpful?

  2. To make a dilution of the stock prepared at 10 mg/ml in HEPES (i-e.10uM which is 34-fold than 2mg/ml) should I still use acidified water, or can i just dilute in PBS or water? This 10 uM dilution will be further dilute 1000 times for cell culture

    1 answer

    1. A stock solution in HEPES at 10 mg/mL, such as that offered as product I0516, may be diluted with water, medium, or the aqueous buffer of choice. This would includes HEPES or PBS. Please see the link below to review the product information sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/132/262/i6634pis.pdf

      Helpful?

  3. How can i make solution of insulin in HEPES buffer and how this solution is storage?

    1 answer

    1. Preparation of Insulin solution: 10 mg/mL insulin in 25 mM HEPES. Recommended storage temperature 2 - 8 °C.

      Helpful?

  4. How can I store the insulin solution?  How long?

    1 answer

    1. The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.

      Helpful?

  5. What can be used to make a solution of insulin?

    1 answer

    1. Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the amino acid sequence for Product I6634, Insulin from bovine pancreas?

    1 answer

    1. The sequence and structure of insulin can be found on the product information sheet (under Documents, above).

      Helpful?

  8. How many units of insulin are present in my vial of Product I6634, Insulin from bovine pancreas?

    1 answer

    1. Product No. I6634 has a specification of not less than 27 USP units/mg.  The exact value for the product received can be found the on lot-specific Certificate of Analysis.

      Helpful?

  9. What is the comparison between International Units (IU) and USP units for insulin?

    1 answer

    1. The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.

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