CLLS1069
MCF10A CELLS APC (-/-)
human female mammary glands (Source Disease: Fibrocystic Disease)
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32190102
Biological source:
human female mammary glands (Source Disease: Fibrocystic Disease)
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This Item | CLLS1211 | CLLS1046 | CLLS1042 |
|---|---|---|---|
| biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... APC(324) | Gene Information human ... PVRL4(81607), nectin-4(81607) | Gene Information human ... PTEN(5728) | Gene Information human ... CDH1(1105) |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
MCF10A CELLS APC (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.
Adenomatous polyposis coli (APC) is a tumor suppressor gene. It is located on human chromosome 5q21.[1]
This cell line corresponds to ATCC Catalog No. CRL-10317.
Adenomatous polyposis coli (APC) is a tumor suppressor gene. It is located on human chromosome 5q21.[1]
Application
Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.
Biochem/physiol Actions
Adenomatous polyposis coli (APC) plays an important role in cell division, adhesion and migration. Mutations in APC gene is associated with colorectal carcinomas.[2]
Features and Benefits
These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 5q21
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Adenomatous polyposis coli (APC) regulates miR17-92 cluster through β-catenin pathway in colorectal cancer.
Li Y, et al.
Oncogene, 35(35), 4558?4568-4558?4568 (2016)
Epigenetic regulation of APC in the molecular pathogenesis of gallbladder cancer.
Tekcham DS, et al.
The Indian Journal of Medical Research, 143(S1), S82?S90-S82?S90 (2016)
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