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76218

Sigma-Aldrich

Papain from Carica papaya

powder, almost white, ~12 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42030311

form

powder

specific activity

~12 U/mg

mol wt

Mr ~23000

color

almost white

storage temp.

−20°C

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Application

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Xiaoqin Wang et al.
Bioresource technology, 126, 307-313 (2012-11-03)
In this study, for the first time, the applications of two new methods, ionic liquid and low-temperature high-pressure cell breakage methods, to the extraction of whole proteins in Chlorella pyrenoidosa cells were explored. Meanwhile, the comparison with three traditional methods
Xu Qin et al.
Chemical communications (Cambridge, England), 49(4), 385-387 (2012-11-30)
A unique route to perfectly alternating oligopeptides is described. First, AG-OEt was prepared by standard chemical coupling. Then, AG-OEt was converted by papain-catalysis in 30 s to (AG)(x) (80%-yield, x = 9.4 ± 0.3). Extension of this strategy to other
Esther E Weinberger et al.
Journal of controlled release : official journal of the Controlled Release Society, 165(2), 101-109 (2012-11-14)
The incidence of allergic disorders and asthma continuously increased over the past decades, consuming a considerable proportion of the health care budget. Allergen-specific subcutaneous immunotherapy represents the only intervention treating the underlying causes of type I allergies, but still suffers
Sappasith Klomklao et al.
Food chemistry, 136(2), 1006-1012 (2012-11-06)
Proteolytic activity of viscera extract from hybrid catfish (Clarias macrocephalus × Clarias gariepinus) was studied. The optimal pH and temperature were 9.0 and 50°C, respectively, when toothed ponyfish (Gazza minuta) muscle was used as a substrate. When viscera extract from
Rashika El Ridi et al.
The Journal of parasitology, 99(2), 194-202 (2012-09-19)
Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH), peroxiredoxin (TPX), and other larval excretory-secretory products (ESP) essentially induce T helper (Th) 1 and Th17 immune responses during a non-protective natural infection. Such an immune environment promotes production of nitric oxide and hydrogen

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