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Key Documents

56821

Sigma-Aldrich

Immersion oil

UV-transparent

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About This Item

MDL number:
UNSPSC Code:
12352200

quality

UV-transparent

color

colorless to almost colorless

refractive index

n20/D 1.517

viscosity

~437 mPa.s(20 °C)

density

1.09 g/mL at 20 °C

fluorescence

λex 200-600 nm; λem 300-800 nm (in accordance)

application(s)

hematology
histology

storage temp.

room temp

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Application

Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. Use immersion oil (56821) together with oil immersion objective lens for high resolution (1000X) light microscopy work.

Caution

may crystallize on storage; can be redissolved on heating to 50°C (1-2 hours)

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Thomas P Burghardt et al.
Applied optics, 48(32), 6120-6131 (2009-11-12)
Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light
P L Appleton et al.
Journal of microscopy, 234(2), 196-204 (2009-04-29)
Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact

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