46955

Fluorescein

for fluorescence, free acid

• Synonym(s): Acid Yellow 73
• Empirical Formula (Hill Notation): C₂₀H₁₂O₅
• CAS Number: 2321-07-5
• Molecular Weight: 332.31
• Colour Index Number: 45350
• Beilstein/REAXYS Number: 94324
• EC Number: 219-031-8
• MDL number: MFCD00005050
• UNSPSC Code: 12171500
• PubChem Substance ID: 329757275
• NACRES: NA.32

Properties

• grade: for fluorescence
• Quality Level: 100
• technique(s): titration: suitable
• fluorescence: λ_ex 490 nm; λ_em 514 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
• InChI key: GNBHRKFJIUUOQI-UHFFFAOYSA-N
• Gene Information: human ... PTPN1(5770) , PTPRF(5792)

Description

General description

Fluorescein dye is the most commonly used fluorescent probe. It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation. Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.

Application

Fluorescein has been used:

• in the permeability assay of Caco-2 cells
• to test its suitability to serve as lifetime fluorescence standards
• as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin
• as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)