70990

α-Cyano-4-hydroxycinnamic acid

matrix substance for MALDI-MS, ≥99.0% (HPLC)

• Synonym(s): α-CCA, α-CHCA, α-Cyano, 4-HCCA, ACCA
• Linear Formula: HOC₆H₄CH=C(CN)CO₂H
• CAS Number: 28166-41-8
• Molecular Weight: 189.17
• Beilstein/REAXYS Number: 3271427
• EC Number: 248-879-1
• MDL number: MFCD00004204
• UNSPSC Code: 12000000
• PubChem Substance ID: 329762519
• NACRES: NA.21

Properties

• grade: matrix substance for MALDI-MS
• Quality Level: 100
• assay: ≥99.0% (HPLC)
• form: solid
• analyte chemical class(es): glycans, peptides, proteins
• technique(s): MALDI-MS: suitable
• mp: 245-250 °C (lit.); 245-250 °C
• solubility: methanol: 10 mg/mL, clear
• cation traces: Ba: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; K: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤10 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• absorption: ≥2000 at 337 nm (mol. absorption)
• SMILES string: OC(=O)\C(=C\c1ccc(O)cc1)C#N
• InChI: 1S/C10H7NO3/c11-6-8(10(13)14)5-7-1-3-9(12)4-2-7/h1-5,12H,(H,13,14)/b8-5+
• InChI key: AFVLVVWMAFSXCK-VMPITWQZSA-N

Description

General description

α-Cyano-4-hydroxycinnamic acid (α-CHCA) is widely used as a matrix in order to facilitate ionization of peptides and proteins in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer.

Application

α-CHCA was used as matrix in improving the detection of low concentration protein digests on MALDI-TOF.

Other Notes

Matrix substance used in MALDI-MS (matrix-assisted laser desorption ionization MS) peptide analysis in proteomics.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1B
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves