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Key Documents

36951

Sigma-Aldrich

6,8-Difluoro-4-methylumbelliferyl phosphate

for fluorescence, ≥98.0% (TLC)

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About This Item

Empirical Formula (Hill Notation):
C10H7F2O6P
CAS Number:
Molecular Weight:
292.13
MDL number:
UNSPSC Code:
12352200

grade

for fluorescence

assay

≥98.0% (TLC)

solubility

DMSO: soluble

fluorescence

λex 366 nm; λem 448 nm in 50 mM phosphate buffer pH 8.0 (alkaline phosphatase)

shipped in

wet ice

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2c(F)c(OP(O)(O)=O)c(F)cc12

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Other Notes

Fluorogenic substrate for the continuous assay of phosphatases[1]

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K R Gee et al.
Analytical biochemistry, 273(1), 41-48 (1999-08-24)
Fluorogenic substrates based on 4-methylumbelliferone (4-MU) have been widely used for the detection of phosphatase and glycosidase activities. One disadvantage of these substrates, however, is that maximum fluorescence of the reaction product requires an alkaline pH, since 4-MU has a

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