Optimized growth conditions for trans-well inserts have not been established. It is suggested to grow these cells on ECM-coated T75 tissue culture flasks as per the user protocol. However, 16HBE14o- cells seem to grow well on fibronectin-coated inserts containing bovine serum albumin. For a detailed protocol reference, the following publication may be useful: Eur J Pharm Biopharm. 2011 Apr;77(3):398-406. An in vitro triple cell co-culture model with primary cells mimicking the human alveolar epithelial barrier. PMID: 21056660.
SCC150
16HBE14o- Human Bronchial Epithelial Cell Line
Human
Synonym(s):
16HBE, 16-HBE, 16HBEo-, 16-HBEo, 16-HBE14o
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
human
Pricing and availability is not currently available.
Product Name
16HBE14o- Human Bronchial Epithelial Cell Line, 16HBE14o- human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR.
biological source
human
technique(s)
cell based assay: suitable, cell culture | mammalian: suitable
General description
16HBE14o- is a human bronchial epithelial cell line isolated from a 1-year old male heart-lung patient and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology, cytokeratin expression, the ability to form tight junctions, and directional ion transport (1). When grown with an air/liquid interface, cilia can be detected. In contrast to most other respiratory cell lines, 16HBE14o- expresses high levels of cystic fibrosis transmembrane conductance regulator (CFTR) mRNA and protein (1). Expression of CFTR is correlated to cAMP-dependent Cl- conductance in a variety of cells, including 16HE14o- epithelial cells.
Application
Biochem/physiol Actions
Epithelial Cells
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Are there any issues with the cell growth of 16HBE14o- cells on 0.4 and 3 micron pore size trans-ell inserts? The cells were seeded at 1x10^5 cells per insert with an area of 0.47 cm^2. After growing the monolayer for two days and removing the medium from the apical side, it has been over a week under ALI conditions, but the cells do not have cilia, and the TEER has not increased.
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