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OP66

Sigma-Aldrich

Anti-Rb (Ab-5) Mouse mAb (LM95.1)

liquid, clone LM95.1, Calbiochem®

Synonym(s):

Anti-Retinoblastoma Associated Protein, Anti-pp105

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

LM95.1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RB1(5925)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~105 kDa Rb protein.
Recognizes the ~105 kDa Rb protein. Sold under license of U.S. Patent 5,853,988 and corresponding patents.
This Anti-Rb (Ab-5) Mouse mAb (LM95.1) is validated for use in Immunoblotting, Paraffin Sections for the detection of Rb (Ab-5).

Immunogen

Epitope: within a 56 kDa C-terminal fragment
Human
recombinant, human Rb

Application

Immunoblotting (1 µg/ml; see application references)

Paraffin Sections (3 µg/ml, see comments and application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
Saos cells
Positive Control
ML-1 cells or normal colon tissue

Other Notes

Dyson, N., et al. 1992. J. Virol.66, 4606.
Dyson, N., et al. 1990. J. Virol.64, 1353.
Horowitz, J.M., et al. 1989. Science243, 937.
Whyte, P., et al. 1988. Nature334, 124.
Klein, G. 1987. Science238, 1539.
Lee, W.H., et al. 1987. Science235, 1394.
Lee, W.H., et al. 1987. Nature329, 642.
Knudson, A.G. 1971. Proc. Natl. Acad. Sci. USA68, 820.
Pre-treatment with heat or pressure cooker may improve staining of some tissue specimens; this should be determined for each specimen. Antibody should be titrated for optimal results in individual systems.

Legal Information

Sold under license of U.S. Patent 5,853,988 and corresponding patents.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Abstracts from USCAP 2019: Genitourinary Pathology (including renal tumors) (776-992).
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(Suppl 2), 1-173 (2019-03-20)
Abstracts from USCAP 2019: Genitourinary Pathology (776-992).
Laboratory investigation; a journal of technical methods and pathology, 99(Suppl 1), 1-173 (2019-03-20)
Nora M Navone et al.
The Prostate, 78(16), 1262-1282 (2018-08-04)
While it has been challenging to establish prostate cancer patient-derived xenografts (PDXs), with a take rate of 10-40% and long latency time, multiple groups throughout the world have developed methods for the successful establishment of serially transplantable human prostate cancer
Michael T Tetzlaff et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(4), 1280-1290 (2018-11-14)
Ocular adnexal (OA) sebaceous carcinoma is an aggressive malignancy of the eyelid and ocular adnexa that frequently recurs and metastasizes, and effective therapies beyond surgical excision are lacking. There remains a critical need to define the molecular-genetic drivers of the
Jeong Namkung et al.
The Journal of clinical endocrinology and metabolism, 97(9), 3224-3230 (2012-07-05)
Müllerian inhibiting substance (MIS) is produced in Sertoli cells of fetal testis and causes regression of müllerian ducts in male embryos. MIS also can induce the cell cycle arrest and apoptosis in müllerian duct-derived tumors in vivo and in vitro.

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