MABF230

Anti-TIM-3, clone RMT3-23, Azide Free Antibody

clone RMT3-23, from rat

• Synonym(s): Hepatitis A virus cellular receptor 2 homolog, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, TIM-3, T-cell membrane protein 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RMT3-23, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_599011
• UniProt accession no.: Q8VIM0
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Havcr2(171285)

Description

General description

The protein called Hepatitis A virus cellular receptor 2 (HAVcr-20), T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3), T-cell immunoglobulin mucin receptor 3 (TIM-3), or T-cell membrane protein 3 and encoded by the human gene HAVCR2/TIM3/TIMD3 is an important protein in the regulation of macrophage activation. TIM-3 is also critical for immune tolerance as it inhibits T-helper type 1 (Th1) mediated auto and alloimmune responses. Finally TIM-3 plays an important role in T-cell homing in the thymus and lymph and is the receptor for the Galectin-9 an important protein modulating cell-cell and cell matrix interactions. TIM-3 is a membrane bound protein that is expressed largely by immune system cells. High TIM-3 expression is also associated with many tumors including renal cancer and acute myelogenous leukemia (AML), and its expression is also a target for anti-cancer therapy as anti-TIM-3 antibodies have been shown to eradicate leukemic stem cells (LSCs) in animal models of AML.

Immunogen

Recombinant protein corresponding to mouse TIM-3.

Application

Inhibits Activity/Function Assay: Co-administration of a representative lot by an independent laboraotory with FVAX significantly suppressed tumor growth to a greater extent than FVAX alone. Treatment with Anti-TIM-3 , clone RMT3-23, Azide Free also augmented antitumor activities of plasmid DNA encoding melanoma target gp100 or TRP2, suggesting that targeting TIM-3 or TIM-4 improves antitumor activiites of other forms of cancer vaccines. (Bagdadi, M., et al. (2013). Cancer Immunol Immunother. 62(4):629-637.).

Flow Cytometry Analysis: 1 µg from a representative lot detected TIM-3 in TIM-3 transfected 1X10E6 BALB/L5178Y cells.

Flow Cytometry Analysis: A representative lot detected TIM-3 in dendritic cells, macrophages, splenocytes, and hepatic mononuclear cells from acute graft-vs-host disease (aGVHD) mice (Oikawa, T., et al. (2006). J Immunol. 177(7):4281-4287.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected TIM-3 in liver tissue from normal and aGVHD mice (Oikawa, T., et al. (2006). J Immunol. 177(7):4281-4287.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected TIM-3 in HMGB1-deficient MEF cells (Chiba, S., et al. (2012). Nat Immunol. 13(9):832-842.).

This Anti-TIM-3 antibody, clone RMT3-23, Azide Free is validated for use in western blotting, flow cytometry, IHC, ICC & Inhibits activity/Function for the detection of TIM-3.

Quality

Evaluated by Western Blotting in TIM-3 transfected L5178Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TIM-3 in 10 µg of TIM-3 transfected L5178Y cell lysate.

Target description

~31 kDa observed

Physical form

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable