MIF, also known as Macrophage migration inhibitory factor, or Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, or Phenylpyruvate tautomerase, and encoded by the gene MIF/GLIF/MMIF, is proinflammatory cytokine involved in the innate immune response to bacterial pathogens. MIF is secreted by macrophages upon stimulation by bacterial lipopolysaccharide or bacterial antigens, and the expression of MIF at sites of inflammation suggests a role as a mediator in regulating the function of macrophages in host defense. MIF also counteracts the anti-inflammatory activity of glucocorticoids and has various enzyme activities but their physiological function is not known. MIF expression is primarily lymphocytes, macrophages and immune system cells. Defects in MIF may be associated with Rheumatoid arthritis systemic juvenile disease, an inflammatory articular disorder with systemic onset beginning at age 16. Also serum levels of MIF are elevated in cases of severe sepsis and are correlated with low survival.
Immunogen
Recombinant protein corresponding to human MIF.
Application
Neutralization Assay Analysis: A representative lot from an independent laboratory detected MIF during the migration of human peripheral blood granulocytes (Rupreht, R. R., et al. (2000). Pflugers Arch. 440(5 Suppl):R78-80.).
This Anti-MIF Antibody, clone M1, preservative free is validated for use in flow cytometry, neutralizing & western blotting for the detection of MIF.
Quality
Evaluated by Flow Cytometry by Jurkat cells.
Flow Cytometry: 1 µg of this antibody detected MIF in 1X10E6 Jurkat cells.
Target description
12 kDa calculated
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Glioblastoma is the most common primary tumor of the brain and has few long-term survivors. The local and systemic immunosuppressive environment created by glioblastoma allows it to evade immunosurveillance. Myeloid-derived suppressor cells (MDSCs) are a critical component of this immunosuppression.
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