MABE1093

Anti-5-Hydroxymethylcytosine (5hmC) Antibody, clone EDL HMC 1A

clone EDL HMC 1A, from mouse

• Synonym(s): 5hmC, 5-hmC
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: EDL HMC 1A, monoclonal
• species reactivity (predicted by homology): all
• technique(s): dot blot: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2bκ
• shipped in: ambient
• target post-translational modification: unmodified

Description

General description

5-Hydroxymethylcytosine (also known as 5-hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC). 5hmC is a DNA pyrimidine nitrogen base and is a predominantly stable DNA modification. It is produced by the action of three Ten-eleven translocation 1 (TET1) enzyme on 5mC. Each mammalian cell contains 5hmC, but its levels vary significantly depending on the cell type. Higher levels of 5hmC are found in neuronal cells and its level is shown to increase with age in hippocampus and cerebellum. It is also prevalent in embryonic stems cells and its reduced levels are shown to result in impaired self-renewal of stem cells. In the brain and in embryonic stem cells, 5hmC in enriched in promoters, gene bodies, and intergenic areas near genes and positively correlates with gene expression at these loci. 5hmC appears to play a role to promote gene expression during active demethylation. Significantly lower of 5hmC have been reported in cancer cell lines, melanoma, adenomas, and carcinomas compared to the normal surrounding tissue.

Specificity

Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).

Target modification is not species-specific

Immunogen

BSA-conjugated 5-hydroxymethylcytidine.

Application

Anti-5-Hydroxymethylcytosine (5hmC), clone EDL HMC 1A, Cat. No. MABE1093, is a highly specific mouse monoclonal antibody that targets DNA with 5-hydroxymethylcytosine (5hmC) modification and has been tested in Dot Blot and Immunoprecipitation.

Dot Blot Analysis: 0.5 µg/mL from a representative lot detected 0.39-100 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated 5 ng of DNA with 5-hydroxymethylcytosine (5hmC) modification, but not DNA with only 5-methylcytosine (5mC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC) modification or unmodified cytosine (Courtesy of Jeong-Heon Lee, Ph.D.; Chad Clark; Tamas Ordog, M.D.; Zhiguo Zhang, Ph.D. Epigenomics Development Laboratory, Epigenomics Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota, USA).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Dot Blot analysis of DNA cytosine modifications.

Dot Blot Analysis: 0.2 µg/mL of this antibody detected 50 ng of DNA with 5-hydroxymethylcytosine (5hmC), but not unmodified DNA or DNA with only 5-methylcytosine (5mC) modification.

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable