MABD55

Anti-TERT (human) Antibody, clone 2C4

clone 2C4, from mouse

• Synonym(s): Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, Short name=TP2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2C4, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_001180305
• UniProt accession no.: O14746
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TERT(7015)

Description

General description

Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of the telomerase. It is also responsible for addition of TTAGGG repeats to the telomere ends of the chromosome, which is important in maintaining cell immortality. In most normal human cells the expression of hTERT is very low. On the other hand, greater than 90 % of human tumors of different pathology and origins have been shown to upregulate telomerase expression, making hTERT a cancer-associated target.

Immunogen

Full length recombinant protein corresponding to human TERT.

Application

Anti-TERT (human) Antibody, clone 2C4 is an antibody against TERT (human) for use in WB, IC & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect TERT in HeLa cells (Masutomi, K., et al. (2003). Cell, Vol. 114:241–253).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect TERT in HeLa cells, BJ and WI-38 fibroblasts (Masutomi, K., et al. (2003). Cell, Vol. 114:241–253).

Quality

Evaluated by Western Blot in human lymph node tissue lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected TERT in 10 µg of human lymph node tissue lysate.

Target description

~90 kDa observed.
Three isoforms exist for human TERT: Isoform 1 at 127 kDa, Isoform 2 at 90 kDa, and Isoform 3 at 120 kDa

Physical form

Format: Purified

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable