MABC523

Anti-BCL7A Antibody, clone 15C

clone 15C, from mouse

• Synonym(s): B-cell CLL/lymphoma 7 protein family member A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15C, monoclonal
• species reactivity: human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001019979
• UniProt accession no.: Q4VC05
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BCL7A(605)

Description

General description

The protein named B-cell CLL/lymphoma 7 protein family member A or BCL7A and encoded by the human gene named BCL7A is protein that interacts with SWI/SNF components (a nucleosome remodeling complex), implicating that BCL7A’s normal function involves chromatin remodeling. BCL7A was original cloned from a chromosomal translocation in a Burkitt lymphoma cell line and appears to be important in B cell malignancy. Overexpression of BCL7A is associated with germinal center phenotype formation in B cells and BCL7A expression is up regulated in CLL and multiple myeloma but lower in mycosis fungoides and peripheral T cell lymphoma. Recent research indicates that BCL7A was expressed at the pro B and T cell stage and persisted through the pre-B and mature B cell stages. BCL7A expression was maintained in late B cell differentiation but down-regulated in late plasma cells and mature T cells. In haematological malignancies, BCL7A protein was present in the majority of precursor and mature B cell lymphomas with increased expression amongst GC-related lymphomas. Mature T cell malignancies did not express BCL7A.

Immunogen

His-tagged recombinant protein corresponding to human BCL7A.

Application

Anti-BCL7A Antibody, clone 15C is a highly specific mouse monoclonal antibody, that targets B-cell CLL/lymphoma 7 protein family member A & has been tested in IHC & Immunofluorescence.

Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected BCL7A in human tonsil tissue (Prof. G. Roncador, CNIO, Madrid).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected BCL7A in human lymph node, spleen, and thymus tissue (Prof. G. Roncador, CNIO, Madrid).

Immunohistochemistry Analysis: A representative lot detected BCL7A in HEK293 cells transfected with BCL7A, and not in HEK293 cells transfected with SPIB protein (Prof. G. Roncador, CNIO, Madrid).

Research Category
Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Immunohistochemistry in human tonsil and colon tissues.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected BCL7A in human tonsil and colon tissues.

Target description

23 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable