MAB3786

Anti-APC Antibody, CT, clone C-APC 28.9

clone C-APC 28.9, Chemicon^®, from mouse

• Synonym(s): Adenomatous Polyposis Coli Protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: C-APC 28.9, monoclonal
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_000038.3
• UniProt accession no.: P25054
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... APC(324) ; mouse ... Apc(11789)

Description

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. C-APC 28.8 can be used for APC expression and detection of APC mutations.

Specificity

Recognizes human APC, molecular weight is approximately 310kDa.

Immunogen

Epitope: C-terminus

Human APC C-terminal fusion with MBP (maltose binding protein).

Application

Anti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western blot

Immunohistochemistry (Frozen sections)

Immunofluorescence

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Quality

Tested

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
POSITIVE CONTROL: colon cell line HCT116

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable