MAB3731

Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877

clone 6AT1877, Chemicon^®, from mouse

• Synonym(s): c-Met, cmet, HGFR, RCCP2, met proto-oncogene (hepatocyte growth factor receptor)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6AT1877, monoclonal
• species reactivity: human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable
• isotype: IgG1
• NCBI accession no.: NM_000245.2
• UniProt accession no.: P08581
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr1234/pTyr1235)
• Gene Information: human ... MET(4233) ; mouse ... Met(17295)

Description

Specificity

Reacts with Met/HGFR (Hepatocyte growth factor receptor) only when phosphorylated at Tyr1234 and Tyr1235. The Met receptor tyrosine kinase is the prototypical member of a small subfamily of growth factor receptors that when activated, induces mitogenic, motogenic, and morphogenic cellular responses. The ligand for Met is hepatocyte growth factor/scatter factor (HGF/SF) and while normal HGF/SF-Met signaling is required for embryonic development, abnormal Met signaling has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes.

Immunogen

Epitope: phospho-specific (Tyr1234/1235)

Synthetic phospho-peptide corresponding to residues surrounding Tyr1234/1235 of human Met.

Application

Anti-Met/HGFR Antibody, phospho-specific (Tyr1234/1235), clone 6AT1877 detects level of Met/HGFR & has been published & validated for use in ELISA.

ELISA (direct): 1:1,000

Optimal working dilutions must be determined by the end user.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Physical form

Format: Purified

Protein G purified immunoglobulin. Liquid in PBS containing 0.09% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable