ABT59
Anti-Pericentrin Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Pericentrin, Kendrin, Pericentrin-B
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PCNT(5116)
General description
Pericentrin binds to calmodulin and is expressed in the centrosome that serves as a multifunctional scaffold for anchoring proteins and protein complexes. Evidence suggests that pericentrin plays a role in microtubule nucleation and spindle organization. Recent studies show pericentrin plays a role in Majewski/micorcephalic osteodysplastic primordial dwarfism type II (MOPDII) and Seckel Syndrome. Recent use in network modeling indicates a direct link between breast cancer susceptibility to centrosome defects. Pericentrin is a potential marker for centrosome defects in cancer and cancer progression, as centrosomal abonormalities in solid tumors are accompanied by increased pericentrin levels and defects in pericentrin organization.
Specificity
Other homologies: Mouse (89% sequence homology). No homology to Rat was found.
Immunogen
KLH-conjugated linear peptide corresponding to human Pericentrin.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Pericentrin in HeLa cells.
This Anti-Pericentrin Antibody is validated for use in WB, IC for the detection of Pericentrin.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Pericentrin in 10 µg of HeLa cell lysate. Western Blot was performed without Tween.
Target description
~250 kDa observed.
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).
Uniprot describes 2 isoforms produced by alternative splicing with molecular weights at 378 & 355 kDa However in endogenous lysates, Pericentrin is observed at ~250 kDa & ~150 kDa for western blots (Wen, Y., et al. (2008). J Biol Chem. 283(5):2793-2803).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Claudia Conte et al.
Biology open, 7(5) (2018-04-25)
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