ABE257
Anti-RAD51 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
DNA repair protein RAD51 homolog 1
Short name=HsRAD51
Short name=hRAD51
Alternative name(s):
RAD51 homolog A
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100 μL
$466.00
$466.00
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
35
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Gene Information
human ... RAD51(5888)
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
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Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| species reactivity rat, human, mouse | species reactivity human, mouse, bovine, rat, guinea pig, rabbit, horse | species reactivity rat, mouse, human | species reactivity human |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
General description
RAD51 is essential for homologous recombination of DNA during repair of double-strand breaks. The N-terminus of RAD51 functions to bind DNA and promote homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). RAD51 is predominantly expressed in the testis and thymus but can also be found in the small intestine, placenta, colon, pancreas and ovaries.
~37 kDa observed. Two isoforms (isoform 1 at 37 kDa and isoform 2 at 26 kDa) produced as a result of alternative splicing are known to exist: .
Immunogen
Histidine-tagged recombinant protein corresponding to human RAD51.
Application
Anti-RAD51 Antibody is a Rabbit Polyclonal Antibody for detection of RAD51 also known as DNA repair protein RAD51 homolog 1, HsRAD51 & has been validated in WB, ICC & IP.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RAD51 in A431 and HeLa cells.
Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.
Immunoprecipitation Analysis: 40 µLof Anti-RAD51 immunoprecipitated RAD51 from HeLa RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Other homologies: Bovine, Equine, and Canine (99% sequence homology).
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1:2,500 dilution of this antibody detected RAD51 on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE515
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zechen Yan et al.
Reproduction (Cambridge, England), 152(5), 431-437 (2016-08-06)
The production of haploid gametes by meiosis is a cornerstone of sexual reproduction and maintenance of genome integrity. Zfp38 mRNA is expressed in spermatocytes, indicating that transcription factor ZFP38 has the potential to regulate transcription during meiosis. In this study
The role of DNA damage and repair in decitabine-mediated apoptosis in multiple myeloma.
Maes, K; De Smedt, E; Lemaire, M; De Raeve, H; Menu, E; Van Valckenborgh, E; McClue et al.
Oncotarget null
Shiny Titus et al.
Science translational medicine, 5(172), 172ra21-172ra21 (2013-02-15)
The underlying mechanism behind age-induced wastage of the human ovarian follicle reserve is unknown. We identify impaired ATM (ataxia-telangiectasia mutated)-mediated DNA double-strand break (DSB) repair as a cause of aging in mouse and human oocytes. We show that DSBs accumulate
Franziska Foertsch et al.
Cell cycle (Georgetown, Tex.), 18(24), 3581-3588 (2019-11-17)
Knowledge about precise numbers of specific molecules is necessary for understanding and verification of biological pathways. The RAD51 protein is central in the repair of DNA double-strand breaks (DSBs) by homologous recombination repair and understanding its role in cellular pathways
Thomas Chabot et al.
Cancers, 11(3) (2019-03-27)
Genomic instability through deregulation of DNA repair pathways can initiate cancer and subsequently result in resistance to chemo and radiotherapy. Understanding these biological mechanisms is therefore essential to overcome cancer. RAD51 is the central protein of the Homologous Recombination (HR)
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