ABE24
Anti-BPTF Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Protein Bptf, Bromodomain PHD-finger Transcription Factor
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BPTF(2186)
mouse ... Bptf(207165)
General description
Nucleosome-remodeling factor subunit BPTF (UniProt A2A654 and E9Q6A7; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen) is encoded by the Bptf (also known as Fac1, Falz) gene (Gene ID 207165) in murine species. BPTF, SNF2L and pRBAP46/48 are the three subunits of the nucleosome-remodelling factor NURF, an ISWI chromatin-remodeling complex that catalyzes ATP-dependent nucleosome sliding. BPTF mediates NURF target site specificity via interactions with transcription factors, histone variants and histone modifications on transcriptionally active genes. BPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity. BPTF is necessary for the survival of c-MYC-overexpressing cells and for c-MYC-driven tumorigenesis in the mouse pancreas. BPTF knockdown leads to decreased c-MYC chromatin accessibility and significantly delays tumour development in pre-neoplastic pancreatic acinar cells. Consistently, BPTF expression in human tumours positively correlates with activation of c-MYC gene signatures.
Specificity
This rabbit polyclonal antibody detected BPTF in wild-type, but not Bptf-knockout mouse cell lysates. A greatly reduced target band was observed using lysates from BPTF shRNA-treated human HepG2 cells (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237; Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).
Immunogen
Epitope: N-terminal region.
GST-tagged recombinant mouse BPTF N-terminal fragment.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
This rabbit polyclonal Anti-BPTF Antibody has been published and validated for use in Immunoprecipitation and Western Blotting applications.
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected BPTF in 50 µg of human HepG2 cell lysate. BPTF shRNA treatment greatly reduced cellular BPTF level (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected BPTF in 50 µg of wild-type, but not Bptf-knockout, mouse embryonic fibroblast (MEF) lysate (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Ctcf and SA2 with BPTF from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Western Blotting Analysis: A representative lot detected BPTF in Ctcf and SA2 immunoprecipitates from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Western Blotting Analysis: A representative lot detected BPTF in ESC, MEF, and CD8+/CD4+ double-positive (DP) thymocytes from wild-type, but not Bptf-knockout, mice (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Note: ABE24 is not recommended for Chromatin Immunoprecipitation (ChIP). For ChIP application, please use ABE1966.
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected BPTF in 50 µg of wild-type, but not Bptf-knockout, mouse embryonic fibroblast (MEF) lysate (Courtesy of Joseph Landry, Ph.D, Virginia Commonwealth University School of Medicine, U.S.A.).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Ctcf and SA2 with BPTF from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Western Blotting Analysis: A representative lot detected BPTF in Ctcf and SA2 immunoprecipitates from mouse embryonic stem cell (mESC) extract (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Western Blotting Analysis: A representative lot detected BPTF in ESC, MEF, and CD8+/CD4+ double-positive (DP) thymocytes from wild-type, but not Bptf-knockout, mice (Qiu, Z., et al. (2015). Mol. Cell. Biol. 35(1):224-237).
Note: ABE24 is not recommended for Chromatin Immunoprecipitation (ChIP). For ChIP application, please use ABE1966.
Quality
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected BPTF in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected BPTF in 10 µg of NIH/3T3 cell lysate.
Target description
~300/250/100 kDa observed. 333.2/321.6 kDa (mouse; UniProt A2A654/E9Q6A7) and 338.3/325.1/322.2 kDa (human isoform 1/2/3; UniProt Q12830) calculated.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Aiman S Alhazmi et al.
The Journal of biological chemistry (2018-08-25)
This article has been withdrawn by Aiman Alhazmi, Marissa Mack, Tiffany Rolle, Jordan Hiegel, Syed Haqqani, Nga Dao, Farheen Zaman, Nak-Kyeong Kim, Neel Scarsdale, Charles Lyons, and Joseph Landry. Some of the genome-wide data sets were flawed and were not analyzed
Nicola Wiechens et al.
PLoS genetics, 12(3), e1005940-e1005940 (2016-03-29)
Within the genomes of metazoans, nucleosomes are highly organised adjacent to the binding sites for a subset of transcription factors. Here we have sought to investigate which chromatin remodelling enzymes are responsible for this. We find that the ATP-dependent chromatin
Ying Li et al.
Nucleic acids research, 44(15), 7173-7188 (2016-05-05)
The modulation of chromatin structure is a key step in transcription regulation in mammalian cells and eventually determines lineage commitment and differentiation. USF1/2, Setd1a and NURF complexes interact to regulate chromatin architecture in erythropoiesis, but the mechanistic basis for this
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