AB3245-I
Anti-TopBP1
from rabbit, purified by affinity chromatography
Synonym(s):
DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, DNA topoisomerase II-binding protein 1, TopBP1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... TOPBP1(11073)
General description
DNA topoisomerase 2-binding protein 1 (UniProt Q92547; also known as DNA topoisomerase II-beta-binding protein 1, DNA topoisomerase II-binding protein 1, TopBP1) is encoded by the TOPBP1 (also known as KIAA0259) gene (Gene ID 11073) in human. Timely DNA replication and accurate chromosome segregation is critical for maintaining genome integrity to avoid cancer and other genetic diseases. TopBP1 plays an important role in mediating DNA processing during mitosis to reduce transmission of DNA damage toG1 daughter cells. Unlike the translesion synthesis scaffold factor Rev1 or K164-ubiquitylated PCNA that serve to recruit translesion polymerases to stalled forks, TopBP1 forms foci upon mitotic entry, marking sites of and promoting unscheduled DNA synthesis at underreplicated regions independent of Rev1 and PCNA. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induces formation of 53BP1 NBs in the next cell cycle. Human TopBP1 contains eight BRCA1 C Terminus (BRCT) domains (a.a. 101-189, 195-284, 354-444, 548-633, 641-738, 900-991, 1259-1351, 1389-1486) and two nuclear localization signal (NLS) motifs (a.a. 852-858, 1517-1520). TopBP1 BRCT1/2-mediated interaction with phosphorylated Rad9 is essential for TopBP1-dependent ATR (ataxia telangiectasia and Rad3-related) activation, while TopBP1 BRCT6 interacts with the phosphorylated transcription factor, E2F1 to repress E2F1 activity in response to DNA damage. BRCT6 is also targeted for poly(ADP-ribosyl)ation by poly(ADP-ribose) polymerase 1 (PARP-1). The innate immune regulator STAT-5 is activated by human papillomavirus (HPV) proteins in HIV-infected cells, resulting in upregulated TopBP1 transcription and ATR pathway activation required for HPV genome amplification.
Specificity
This rabbit polyclonal antibody targets human TopBP1 region between the sixth and seventh BRCA1 C-terminus (BRCT) domain.
Immunogen
KLH-conjugated linear peptide corresponding to a squence within the region between BRCT domain 6 and 7 of human TopBP1.
Application
Anti-TopBP1 Antibody, Cat. No. AB3245-I, is a highly specific rabbit polyclonal antibody that targets DNA topoisomerase 2-binding protein 1 (TopBP1) and has been tested in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated TopBP1 from 500 µg of HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected TopBP1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected TopBP1 in 10 µg of HeLa cell lysate.
Target description
~180 kDa observed. 170.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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