AB3096
Anti-Orexin Antibody, Prepro
Chemicon®, from rabbit
Synonym(s):
Anti-NRCLP1, Anti-OX, Anti-PPOX
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PPOX(5498)
Specificity
Mouse Prepro-Orexin. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
Immunogen
A 17 mer peptide (designated PPOXA11-A) mapping near the C-terminus of mouse prepro-Orexin.
Application
Anti-Orexin Antibody, Prepro is an antibody against Orexin for use in ELISA, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Circadian Rhythm & Sleep
CNS Control of Metabolism
Circadian Rhythm & Sleep
Western blot: 1-10 μg/mL using Chemiluminescence technique
ELISA: 1:10,000 - 100,000
Immunohistochemistry: 2-20 μg/mL.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000 - 100,000
Immunohistochemistry: 2-20 μg/mL.
Optimal working dilutions must be determined by end user.
Physical form
Liquid in PBS with 0.1% BSA.
Storage and Stability
Can be refrigerated at 2-8°C in undiluted aliquots for short term use. Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Luigi Michele Pavone et al.
Anatomical record (Hoboken, N.J. : 2007), 292(2), 202-206 (2008-12-18)
The hypothalamic peptide orexin A (oxA) binds specifically the G-protein-coupled orexin receptor 1 (ox1R). It is involved in many physiological functions including the regulation of food intake, sleep-wake cycle, arterial blood pressure, heart rate, and sexual behavior. The localization of
Shuqin Zhan et al.
Journal of cellular and molecular medicine, 23(10), 6822-6834 (2019-08-07)
Hypocretin 1 and hypocretin 2 (orexin A and B) regulate sleep, wakefulness and emotion. Tumour necrosis factor alpha (TNF-α) is an important neuroinflammation mediator. Here, we examined the effects of TNF-α treatment on hypocretin expression in vivo and behaviour in
E Schéle et al.
Journal of neuroendocrinology, 24(6), 930-943 (2012-02-03)
Interleukin (IL)-6 deficient mice develop mature-onset obesity. Furthermore, i.c.v. administration of IL-6 increases energy expenditure, suggesting that IL-6 centrally regulates energy homeostasis. To investigate whether it would be possible for IL-6 to directly influence the energy homeostasis via hypothalamic regulation
G Liguori et al.
Anatomia, histologia, embryologia, 43(1), 42-47 (2013-03-26)
Orexins A (ox A) and B are two peptides originally discovered in neurons of rat hypothalamus, and later found in different cellular types of the gastrointestinal and genital tracts. They arise from the proteolytic cleavage of a common precursor molecule
Ming-Fung Wu et al.
Sleep, 46(9) (2023-05-08)
Long-term use of sodium oxybate (SXB), (also called gamma-hydroxybutyrate [GHB]) attenuates the cataplexy and sleepiness of human narcolepsy. We had previously found that chronic opiate usage in humans and long-term opiate administration to mice significantly increased the number of detected
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