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biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Specificity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
Immunogen
Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
Application
Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified
Storage and Stability
The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Journal of immunology (Baltimore, Md. : 1950) (1950)
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Takeo Kitamoto,Kazunori Hanaoka
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Ottaviano, AJ; Sun, L; Ananthanarayanan, V; Munshi, HG
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Selective matrix metalloproteinase inhibition with developing heart failure: effects on left ventricular function and structure.
Mary K King, Mytsi L Coker, Aaron Goldberg, James H McElmurray, Himali R Gunasinghe et al.
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George C Engelmayr, Virna L Sales, John E Mayer, Michael S Sacks
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