MABT530-AF488

Anti-N-cadherin Antibody, CT Antibody, clone 6A9.2 Antibody, Alexa Fluor^™ 488 Conjugate

clone 6A9.2, from mouse, ALEXA FLUOR^™ 488

• Synonym(s): CD325, CDw325, cadherin-2, Neural cadherin
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6A9.2, monoclonal
• species reactivity: rat, human
• technique(s): immunocytochemistry: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001783
• UniProt accession no.: P19022
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CHD2(1106)

Description

General description

Cadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. N-Cadherin is a single-pass type I membrane protein that plays a role in calcium-dependent cell adhesion. N-cadherins preferentially interacts with themselves in a homophilic manner in connecting cells; may thus contribute to the sorting of heterogeneous cell types. Cadherins are synthesized as precursor proteins that are proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. N-cadherin is well expressed in endothelial cells and plays an essential role in the maturation and stabilization of normal vessels. However, it also promotes tumor cell survival, migration and invasion, and a high level of its expression is often associated with poor prognosis.

Specificity

Clone 6A9.2 specifically detects N-cadherin extracellular cadherin 1 domain. It targets an epitope with in a sequence of 79 amino acids in the N-terminal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 79 amino acids from the N-terminal half of human cadherin-2.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected N-Cadherin in HeLa cells.

The unconjugated antibody (Cat. No. MABT530) is shown to be suitable also for Western blotting and Immunohistochemistry (Paraffin) applications.

Research Category
Cell Structure

This mouse monoclonal Anti-N-cadherin, CT Antibody, clone 6A9.2, Alexa Fluor^™ 488 Conjugate, Cat. No. MABT530-AF488 is tested in Immunocytochemistry for the detection of N-Cadherin.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected N-Cadherin in A431 cells.

Target description

99.81 kDa calculated.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS with 1.5% BSA and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable