MABN609

Anti-OCRL/INPP5b Antibody, clone N166A/26

clone N166A/26, from mouse

• Synonym(s): Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: N166A/26, monoclonal
• species reactivity: human, rat
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000267
• UniProt accession no.: Q01968
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... OCRL(4952)

Description

General description

Inositol polyphosphate 5-phosphatase OCRL-1 (ORCL) is also called Lowe oculocerebrorenal syndrome protein. ORCL is a phosphotase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. OCRL defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).

Specificity

Cross-reacts with OCRL/INPP5b (Prof. J. Trimmer, Univerity of California, Davis).

Immunogen

Recombinant protein corresponding to human OCRL/INPP5b.

Application

Detect Inositol polyphosphate 5-phosphatase OCRL-1 using this mouse monoclonal antibody, Anti-OCRL/INPP5b Antibody, clone N166A/26 validated for use in western blotting & IHC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected OCRL/INPP5b in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected OCRL/INPP5b in COS cell lysates transiently transfected with GFP-tagged OCRL or RFP-tagged INPP5b, and demonstrated a loss of signal in COS cell lysates transiently transfected with untagged Kv2.1 plasmids (Prof. J. Trimmer, University of California, Davis.).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OCRL/INPP5b in human cerebellum and human cardiac myocytes.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected OCRL/INPP5b in 10 µg of human brain tissue lysate.

Target description

~104 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable