Numb plays a key role in neurogenesis and in cell fate decisions. It maintains self-renewal properties in neural progenitor cells, which are also called radial glial cells (RGCs). It functions through inhibition of Notch signaling. Additionally, it is suggested to mediate local repair in brain ventricular wall damage.
Specificity
This antibody recognizes Numb isoforms 1 and 2.
Immunogen
KLH-conjugated linear peptide corresponding to human Numb isoforms 1 and 2.
Application
Detect Numb using this Anti-Numb Antibody, clone 6G10.1 validated for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Numb in HeLa and A431 cells.
Research Category Neuroscience
Research Sub Category Developmental Neuroscience
Quality
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Numb in 10 µg of A431 cell lysate.
Target description
~70 kDa observed. Uniprot describes four isoforms at ~71 kDa (isoform 1), ~66 kDa (isoform 2), ~69 kDa (isoform 3), and ~65 kDa (isoform 4).
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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