MABD401
Anti-Neural Stem Cells Antibody, clone Nilo1
clone Nilo1, from hamster(Armenian)
Synonym(s):
Neural stem cells, Neural progenitors, NSCs, Type B astrocytes
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
biological source
hamster (Armenian)
antibody form
purified antibody
antibody product type
primary antibodies
clone
Nilo1, monoclonal
species reactivity
mouse, human
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
magnetic resonance imaging (MRI): suitable
western blot: suitable
shipped in
ambient
target post-translational modification
unmodified
General description
Similar to other cell types, neural cells are generated from primary progenitor cells known as type B astrocytes or neural stem cells (NSCs) via stages of amplification (transient amplifying cells), resulting in the generation of intermediate precursor cells (iPCs) committed to neurons (nIPCs), astrocytes (aIPCs), or oligodendrocytes (oIPCs). In adult rodent brain, NSCs and iPCs are mainly restricted to niches in the subventricular zone (SVZ) of the lateral ventricles (LV). In the adult SVZ, type B cells (SVZB) generate neuroblasts (type A cells, neuronal precursors) through a highly proliferative transit amplifying population (type C cells). NSCs and iPCs paly important roles in neurogenesis in the adult brain. Neuroblasts, for example, are known to migrate from their SVZ niche through the rostral migratory stream (RMS) to the olfactory bulb for the continuous generation of periglomerular interneurons. In addition to maintaining the homeostasis of the olfactory bulb, both neuroblasts and NSCs are shown to mobilize from their SVZ niches to various brain damage sites caused by tumor growth, cryolesion, focal demyelinization, and mechanical lesion.
Specificity
Nilo (Neural Identification Lineage from Olfactory bulb) monoclonal antibody Nilo1 (Cat. No. MABD401) recognizes neural stem cells (NSCs; type B astrocytes) in adult mouse brain and radial glia in mouse embryo (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129), while Nilo2 (Cat. No. MABD402) targets neuroblasts (Elvira, G., et al. (2012). PLoS One. 7(9):e44466). Clone Nilo1 identified early progenitor cells (Sox2+, EGFR+, GFAP+ and vimentin+), whereas Nilo2 identified more differentiated neural progenitor cells committed to the neuroblast pathway (Tuj-1+, PSA-NCAM+ and DCX+). Nilo1 and Nilo2 arrest neurosphere proliferation in vitro and interfered with their differentiation into mature neural cells by targeting different surface glycoproteins highly relevant on neural stem/early progenitor cell biology (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunogen
E13.5 FVB mouse embryo olfactory bulb-derived neurospheres (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Application
Magnetic Resonance Imaging (MRI) Analysis: A representative lot, when coupled to magnetic glyconanoparticles (mGNPs) and injected in mice, allowed the tracking of neural stem cells migrating from their niches towards brain injury sites by MRI (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Flow Cytometry Analysis: A representative lot, coupled to magnetic glyconanoparticles (mGNPs) or not, immunostained the surface of SVZ neurosphere-derived single cells (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Flow Cytometry Analysis: Clone Nilo1 hybridoma culture supernatant stained the surface of 61% and 71% neurosphere-derived cells from mouse embryo olfactory bulb (OB) and adult mouse subventricular zone (SVZ), respectively (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed primary human glioblastoma grown in culture as neurospheres (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Immunocytochemistry Analysis: Representative lots immunostained early neural precursor cells by fluorescent immunocytochemistry staining of cultured neurospheres or neurosphere-derived single cells fixed with 4% paraformaldehyde. A loss of Nilo1 immunoreactivity was observed within 7 days following differentiation induction by EGF and bFGF withdrawal in neurosphere cultures (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunofluorescence Analysis: A representative lot, when injected in mice, allowed the labelling and detection of undifferentiated neural stem cells migrating towards brain injury sites due to CT-2A tumor growth, cryolesion, demyelination, or mechanical damage via stereotaxic PBS injection (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Immunofluorescence Analysis: Representative lots immunostained quiescent neural progenitor cells, whereas Nilo2 (Cat. No. MABD402) stained post-mitotic neuronal precursors (e.g. type 1 neuroblasts) in subventricular zone (SVZ) by fluorescent immunohistochemistry using 4% paraformaldehyde-fixed adult mouse floating sections (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunohistochemistry Analysis: A representative lot immunostained a small cell population lining the SVZ ventricle, distinct from the thin layer of cells stained by Nilo2 (Cat. No. MABD402) in the periventricular area inside the anterior SVZ (SVZa), at the beginning of the RMS. Both Nilo1 and Nilo2 stained ependymal and subependymal layers in adult mouse olfactory bulb core, neither clone stained dentate gyrus (DG) of the hippocampus (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunoprecipitation Analysis: A representative lot immunoprecipitated a ~40 kDa glycoprotein from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Inhibition Analysis: A representative lot inhibited the differentiation of adult mice SVZ-derived neurospheres in culture as well as the proliferation of neurosphere-derived single cells (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Western Blotting Analysis: A representative lot detected a ~40 kDa glycoprotein in the immunoprecipitates obtained by clone Nilo1 from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Flow Cytometry Analysis: A representative lot, coupled to magnetic glyconanoparticles (mGNPs) or not, immunostained the surface of SVZ neurosphere-derived single cells (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Flow Cytometry Analysis: Clone Nilo1 hybridoma culture supernatant stained the surface of 61% and 71% neurosphere-derived cells from mouse embryo olfactory bulb (OB) and adult mouse subventricular zone (SVZ), respectively (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed primary human glioblastoma grown in culture as neurospheres (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Immunocytochemistry Analysis: Representative lots immunostained early neural precursor cells by fluorescent immunocytochemistry staining of cultured neurospheres or neurosphere-derived single cells fixed with 4% paraformaldehyde. A loss of Nilo1 immunoreactivity was observed within 7 days following differentiation induction by EGF and bFGF withdrawal in neurosphere cultures (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunofluorescence Analysis: A representative lot, when injected in mice, allowed the labelling and detection of undifferentiated neural stem cells migrating towards brain injury sites due to CT-2A tumor growth, cryolesion, demyelination, or mechanical damage via stereotaxic PBS injection (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).
Immunofluorescence Analysis: Representative lots immunostained quiescent neural progenitor cells, whereas Nilo2 (Cat. No. MABD402) stained post-mitotic neuronal precursors (e.g. type 1 neuroblasts) in subventricular zone (SVZ) by fluorescent immunohistochemistry using 4% paraformaldehyde-fixed adult mouse floating sections (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunohistochemistry Analysis: A representative lot immunostained a small cell population lining the SVZ ventricle, distinct from the thin layer of cells stained by Nilo2 (Cat. No. MABD402) in the periventricular area inside the anterior SVZ (SVZa), at the beginning of the RMS. Both Nilo1 and Nilo2 stained ependymal and subependymal layers in adult mouse olfactory bulb core, neither clone stained dentate gyrus (DG) of the hippocampus (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Immunoprecipitation Analysis: A representative lot immunoprecipitated a ~40 kDa glycoprotein from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Inhibition Analysis: A representative lot inhibited the differentiation of adult mice SVZ-derived neurospheres in culture as well as the proliferation of neurosphere-derived single cells (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Western Blotting Analysis: A representative lot detected a ~40 kDa glycoprotein in the immunoprecipitates obtained by clone Nilo1 from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Research Category
Stem Cell Research
Stem Cell Research
This hamster monoclonal Anti-Neural Stem Cells Antibody, clone Nilo1, Cat. No. MABD401, is validated for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Magnetic Resonance Imaging, Inhibition, and Western Blotting.
Quality
Evaluated by Immunohistochemistry in C57BL/6 mouse brain sections.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody immunostained neural stem cells in subventricular zone (SVZ) of the lateral ventricle (LV) in 4% paraformaldehyde-fixed free-floating C57BL/6 mouse brain sections.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody immunostained neural stem cells in subventricular zone (SVZ) of the lateral ventricle (LV) in 4% paraformaldehyde-fixed free-floating C57BL/6 mouse brain sections.
Target description
~40 kDa (glycosylated) reported. (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
Physical form
Format: Purified
Protein G purified.
Purified Armenian hamster monoclonal antibody in PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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