Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.
Specificity
Other species have not been tested.
The antibody reacts with the 22 kDa phospho-Bid-S65 protein.
Immunogen
Epitope: Ser65
Application
Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.
Research Category Apoptosis & Cancer
Research Sub Category Apoptosis - Additional
Quality
Evaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.
Western Blot Analysis: A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.
Target description
22 kDa
Physical form
Antigen Affinity Purified
Purified in PBS with 0.09% NaN3.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control Western Blot:: Jurkat cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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