Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Specificity
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins collagen and non-collagen extracellular matrix proteins to remove unwanted reactivities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or fibronectin. Non-specific cross reaction of anti-laminin antibodies with other human serum proteins or non-laminin extracellular matrix proteins is negligible.
Immunogen
Full length human laminin. Immunization occurred after single-band purity was assessed by SDS-PAGE.
Application
Detect Laminin using this Anti-Laminin Antibody validated for use in ELISA, IP, WB, IH.
Immunoblot: 1:500-1:2000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Immunoprecipitation: Condition must be determined by the end user
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid at 1 mg/mL in 0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 with 0.01% Sodium Azide.
Format: Purified
Storage and Stability
The undiluted antibody solution is stable for approximately 6 months stored between 2 and 8° C. For extended storage mix with an equal volume of glycerol, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Dhruv Sareen et al.
PloS one, 4(10), e7630-e7630 (2009-11-10)
Stem cell expansion and differentiation is the foundation of emerging cell therapy technologies. The potential applications of human neural progenitor cells (hNPCs) are wide ranging, but a normal cytogenetic profile is important to avoid the risk of tumor formation in
MMP-14 and TIMP-2 overexpression protects against hydroquinone-induced oxidant injury in RPE: implications for extracellular matrix turnover.
Alcazar, O; Cousins, SW; Marin-Casta?o, ME
Investigative Ophthalmology & Visual Science null
Cathepsin-L influences the expression of extracellular matrix in lymphoid organs and plays a role in the regulation of thymic output and of peripheral T cell number.
Gabriela Lombardi, Dalia Burzyn, Juliana Mundi?ano, Paula Berguer, Pedro Bekinschtein et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
Notch3 null mutation in mice causes muscle hyperplasia by repetitive muscle regeneration.
Takeo Kitamoto,Kazunori Hanaoka
Stem Cells null
Extracellular matrix-mediated membrane-type 1 matrix metalloproteinase expression in pancreatic ductal cells is regulated by transforming growth factor-beta1.
Ottaviano, AJ; Sun, L; Ananthanarayanan, V; Munshi, HG
Cancer Research null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service