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14-1053

Sigma-Aldrich

Human recombinant histone H2AZ-H2B dimers

Human recombinant histone H2AZ-H2B dimers expressed in E Coli as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, & purified by Fast Protein Liquid Chromatography (FPLC)& verified by mass spectrometry.

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
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biological source

Escherichia coli

recombinant

expressed in E. coli

assay

>98% (SDS-PAGE)

manufacturer/tradename

Upstate®

concentration

1 μg/μL

technique(s)

activity assay: suitable

shipped in

dry ice

General description

Human recombinant histone H2AZ-H2B dimers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Product Source: Human
The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The H2AZ histone variant replaces conventional H2A in a subset of nucleosomes and plays an essential role in transcriptional regulation, autosilencing, gene silencing, and genome stability. The effects of histone variants and histone modifications on chromatin structure and gene expression can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin and essential tools, such as histone proteins and appropriate enzymes to assemble chromatin in vitro.

Application

Research Category
Epigenetics & Nuclear Function
Suitable for in vitro chromatin assembly and enzyme activity assays

Physical form

In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 1 M NaCl, 0.5 mM PMSF and 1 mM DTT

Storage and Stability

Stable at -80ºC for 1 year from date of receipt.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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