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1.24653

Sigma-Aldrich

4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI)

for microscopy

Synonym(s):

4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI)

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About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
MDL number:
UNSPSC Code:
41116130
EC Index Number:
249-186-7

assay

≥98% dry basis

form

solid

pH

4.0-5.0 (20 °C, 10 g/L in H2O)

mp

330 °C (decomposition)

bulk density

120 kg/m3

εmax

800-900 at 340-343 nm at 0.0005% (pH 7.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

4′,6-Diamidino -2-phenylindole dihydrochloride is better known as "DAPI". It is a dry dye used for the preparation of a color staining solution that can be applied for fluorescence staining of DNA in nuclei in vivo or as counter stain in immunofluorescent methods in sample material of human origin.
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Analysis Note

Assay (argentometric; calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (buffer pH 7.0): 340 - 343 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.0005 %; buffer pH 7.0; calc. on dried substance): 800 - 900
Loss on drying (105 °C): ≤ 5 %

Certificates of Analysis (COA)

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Santos Blanco et al.
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