1.15931

Acridine orange zinc chloride double salt (C.I.46005)

for microscopy Certistain^®

• Synonym(s): Acridine orange zinc chloride double salt (C.I.46005)
• Empirical Formula (Hill Notation): C₁₇H₂₀Cl₃N₃Zn
• CAS Number: 10127-02-3
• Molecular Weight: 438.11
• MDL number: MFCD00081043
• UNSPSC Code: 41116130
• EC Index Number: 233-353-6

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥50% spectrophotometric assay
• pH: 3.9 (20 °C, 10 g/L in H₂O)
• mp: 165 °C
• solubility: 28 g/L
• bulk density: 200 kg/m³
• εmax: ≥1120 at 491-495 nm in 50% ethanol at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C17H19N3.3ClH.Zn/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;/h5-11H,1-4H3;3*1H;/q;;;;+2/p-2
• InChI key: VADJQOXWNSPOQA-UHFFFAOYSA-L

Description

General description

Acridine orange zinc chloride double salt (C.I. 46005) for microscopy Certistain^®, is a< dry dye that is used for the preparation of a staining solution for fluorescence staining for differential staining of DNA and RNA in sample material of human origin. As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 50% C₁₇H₂₀ClN₃
Identity
- chloride:
passes test
- UV/VIS-Spectrum: passes test
- Zinc: passes test
Absorption maximum λ max (ethanol 50 %): 491 - 495 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; ethanol 50 %): ≥ 1120
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3