07-1229
Anti-DAPK2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
DAP kinase, DAP- kinase-related protein, Death-associated protein kinase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DAPK2(23604)
rat ... Dapk2(300799)
General description
Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.
Specificity
Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.
Immunogen
Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.
Application
Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).
Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Quality
Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
Target description
Detects a 42 kDa band by western blot.
Linkage
Replaces: AB3606
Physical form
Affinity purified
Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.
Storage and Stability
Stable for 6 months at 2-8° C from date of receiept.
Analysis Note
Control
C2C12 cell lysate
C2C12 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Snehal Dabir et al.
International journal of cancer, 134(5), 1045-1054 (2013-08-21)
Protein inhibitor of activated signal transducer and activator of transcription 3 (STAT3) (PIAS3) is an endogenous inhibitor of STAT3 that negatively regulates STAT3 transcriptional activity and cell growth and demonstrates limited expression in the majority of human squamous cell carcinomas
B Inbal et al.
Molecular and cellular biology, 20(3), 1044-1054 (2000-01-11)
In this study we describe the identification and structure-function analysis of a novel death-associated protein (DAP) kinase-related protein, DRP-1. DRP-1 is a 42-kDa Ca(2+)/calmodulin (CaM)-regulated serine threonine kinase which shows high degree of homology to DAP kinase. The region of
T Kawai et al.
Oncogene, 18(23), 3471-3480 (1999-06-22)
We have identified and characterized a new calcium/calmodulin (Ca2+/CaM) dependent protein kinase termed death-associated protein kinase 2 (DAPK2) that contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a
Lidia Villanova et al.
International journal of molecular sciences, 21(7) (2020-04-05)
MicroRNAs are tiny but powerful regulators of gene expression at the post-transcriptional level. Aberrant expression of oncogenic and tumor-suppressor microRNAs has been recognized as a common feature of human cancers. Colorectal cancer represents a major clinical challenge in the developed
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