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04-742

Sigma-Aldrich

Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal

culture supernatant, clone NL213, Upstate®

Synonym(s):

Anti-DRPLA

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

NL213, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002831
NM_002834

UniProt accession no.

P29350

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PTPN6(5777)
mouse ... Ptpn6(15170)

Specificity

Others not tested.
Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa

Immunogen

Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria

Application

Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
This Anti-SHP-1/2 Antibody, clone NL213 is validated for use in IP, WB for the detection of SHP-1/2.

Quality

routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells

Target description

~65kDa

Linkage

Replaces: 05-742

Physical form

Cultured supernantant in 0.05% sodium azide

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nicolas Drapeau et al.
American journal of physiology. Endocrinology and metabolism, 304(11), E1188-E1198 (2013-03-28)
Renal podocyte apoptosis is an early event of diabetic nephropathy progression. Insulin action is critical for podocyte survival. Previous studies demonstrated that Src homology-2 domain-containing phosphatase-1 (SHP-1) is elevated in renal cortex of type 1 diabetic mice; we hypothesized that
Pawan Kumar Raghav et al.
PloS one, 13(11), e0206364-e0206364 (2018-11-06)
Enhancement of hematopoietic stem cells (HSCs) proliferation is a central aim in bone marrow transplantation (BMT). A stem cell factor (SCF) and c-Kit mediated extracellular signaling trigger proliferation of HSCs. This signaling is negatively regulated by protein tyrosine phosphatases (PTPs)

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