V900891
Collagenase from Clostridium histolyticum
Type I, for general use, Vetec™, reagent grade, powder, ≥125 CDU/mg solid
Synonym(s):
Clostridiopeptidase A
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About This Item
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Recommended Products
grade
reagent grade
product line
Vetec™
form
powder
specific activity
≥125 CDU/mg solid
storage temp.
−20°C
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Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. VETEC Collagenase Type I has the original balance of collagenase, caseinase, clostripain, and tryptic activities, and is suggested for epithelial, liver, lung and adrenal primary cell isolations.
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Quality
Also contains clostripain, nonspecific neutral protease, and tryptic activities.
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Legal Information
Vetec is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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