845123

TOYOPEARL^® Phenyl-600M MiniChrom Column

phase phenyl, 65 μm particle size, pore size 750 Å, L × I.D. 10 cm × 8 mm, MiniChrom Column

• Synonym(s): MiniChrom
• UNSPSC Code: 41115700

Properties

• material: PEEK
• product line: TOYOPEARL^®
• analyte chemical class(es): proteins
• manufacturer/tradename: Tosoh 45123
• parameter: ≤50% organic solvent; 3 bar max. pressure (resin); 30 bar max. pressure (column)
• technique(s): HPLC: suitable; LPLC: suitable
• L × I.D.: 10 cm × 8 mm
• bed volume: 5 mL
• matrix: hydroxylated methacrylic polymer
• matrix active group: phenyl phase
• particle size: 65 μm
• pore size: 750 Å
• operating pH: 1-13
• capacity: 55 g/L±10 adsorption capacity (lysozyme)
• separation technique: hydrophobic interaction (HIC)

Description

General description

MiniChrom columns for TOYOPEARL^® and TSKgel^® are pre-packed with the most popular ion exchange, mixed-mode, hydrophobic interaction or affinity media. These columns are available in 5 mL volume, 8 mm ID x 10 cm L, and can be operated with standard laboratory LC systems. MiniChrom columns for TOYOPEARL and TSKgel provide:

• Direct connection to common laboratory LC Systems
• Easy parameter screening and method optimization
• Fast small scale purification
• Reliable and reproducible results

Toyopearl Butyl-600 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is between that of Toyopearl Butyl-650 and Toyopearl SuperButyl-550 resin. It has been optimized for the purification of monoclonal antibodies and is typically used as an interresinte purification or polishing step.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol, 0.15 M NaCl.

Other Notes

Columns are tolerant to aqueous buffers and salt solutions, 1 mol/L alkaline solutions, 1 mol/L non-oxidizing mineral acids, 8 mol/L urea, 6 mol/L guanidine hydrochloride, non-halogenated organic solvents and detergents. They are not compatible with strong oxidants and halogenated solvents.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation