823432

ToyoScreen^® AF-rProtein A HC-650F Process Development Column

phase rProtein A, pkg of 5 × 5 mL, 45 μm particle size

• UNSPSC Code: 41106500

Properties

• material: aluminum seal; polypropylene and polyethylene frit
• product line: ToyoScreen^®
• specific analyte(s): IgG
• packaging: pkg of 5 × 5 mL
• manufacturer/tradename: Tosoh 23432
• parameter: 1-5 mL/min flow rate; 20 mL/min max. flow rate; 3 bar max. pressure
• technique(s): HPLC: suitable
• L × I.D.: 3 cm × 14.6 mm
• matrix: hydroxylated methacrylic polymer
• matrix active group: rProtein A phase
• particle size: 45 μm
• operating pH: 2-13
• capacity: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• separation technique: affinity

Description

General description

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen^® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl^® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

Specifications

Clean in place with 0.1 M NaOH.

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Legal Information

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation