821870

ToyoScreen^® MegaCap II SP-550EC Process Development Column

phase sulfonic acid, pkg of 6 × 1 mL, 200 μm particle size

• UNSPSC Code: 41115700

Properties

• material: aluminum seal; polypropylene and polyethylene frit
• product line: ToyoScreen^®
• feature: counter ion Na+
• analyte chemical class(es): peptides
• packaging: pkg of 6 × 1 mL
• manufacturer/tradename: Tosoh 21870
• parameter: 0.2-1 mL/min flow rate; 3 bar max. pressure; 4 mL/min max. flow rate
• technique(s): HPLC: suitable
• L × I.D.: 3 cm × 6.4 mm
• matrix: hydroxylated methacrylic polymer
• matrix active group: sulfonic acid phase
• particle size: 200 μm
• operating pH: 2-13
• capacity: 127.5 g/L±27.5 adsorption capacity (insulin)
• separation technique: cation exchange

Description

General description

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen^® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl^® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

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Legal Information

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation