Unlike other TSKgel affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups. The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.
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