814443

TSKgel^® Heparin-5PW HPLC Column

phase sulfated glycosaminoglycan, L × I.D. 5 cm × 5 mm, 10 μm particle size

• UNSPSC Code: 41115711

Properties

• material: glass
• product line: TSKgel^®
• analyte chemical class(es): proteins
• manufacturer/tradename: Tosoh 14443
• parameter: ≤20% organic solvent; ≤3 M salt concentration; 1.0 mL/min flow rate; 20 bar max. pressure; 4-30 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 5 cm × 5 mm
• matrix: hydroxylated methacrylic polymer
• matrix active group: sulfated glycosaminoglycan phase
• particle size: 10 μm
• operating pH: 5.5-9.0
• capacity: >1.8 mg/mL, resin adsorption capacity (antithrombin III)
• separation technique: affinity
• storage temp.: 2-8°C

Description

General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

The heparin ligand in TSKgel Heparin-5PW affinity columns can interact with sample molecules by cation exchange as well as affinity chromatography. Cation exchange interaction takes place through O-sulfate and N-sulfate groups. The elution strength is similar to TSKgel SP-5PW.

In vivo, heparin prevents blood clotting as it reacts with clotting agents. This tendency is taken advantage of by using heparin to separate blood clotting factors such as antithrombin III, Factor VII, Factor IX,etc., and anti-heparin proteins such as glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, RNA polymerases, and other nucleic acid-binding proteins.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation