814440
TSKgel® Chelate-5PW (10 µm) HPLC Columns
L x I.D. 5 cm × 5 mm, iminodiacetic acid phase
Synonym(s):
TSKgel® Chelate-5PW HPLC Column and Guardgel Kit
Select a Size
$1,630.00
Notify Me
Get notified when this item is ready to ship via email.
Select a Size
About This Item
$1,630.00
Notify Me
Get notified when this item is ready to ship via email.
Product Name
TSKgel® Chelate-5PW HPLC Column, phase iminodiacetic acid, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
material
glass
product line
TSKgel®
manufacturer/tradename
Tosoh 14440
parameter
≤20% organic solvent
≤4 M salt concentration
≤45 °C temperature
1.0 mL/min flow rate
20 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
5 cm × 5 mm
matrix
hydroxylated methacrylic polymer
matrix active group
iminodiacetic acid phase
particle size
10 μm
operating pH
2-12
separation technique
affinity
Looking for similar products? Visit Product Comparison Guide
General description
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
Legal Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.
If you need assistance, please contact Customer Support
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
