807476
TOYOPEARL® Butyl-650S Bulk Media
phase C4 (butyl), bottle of 100 mL, 35 μm particle size
Synonym(s):
TOYOPEARL® Butyl-650 HIC Bulk Media
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UNSPSC Code:
23151817
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description
Butyl-650S
product line
TOYOPEARL®
form
slurry
packaging
bottle of 100 mL
parameter
3 bar max. pressure
technique(s)
LPLC: suitable
matrix
polymer (35 μm)
matrix active group
C4 (butyl) phase
particle size
35 μm
operating pH
1-13
capacity
40 g/L±10 adsorption capacity (lysozyme)
separation technique
hydrophobic interaction (HIC)
General description
TOYOPEARL® HIC resins are hydrophobic interaction chromatography resins that offer the following advantages: strong affinity for water-soluble proteins, high recovery of mass and activity, high sample capacity up to 2-4 times that of gel media, fluctuating salt concentrations will not change the bed volume, mechanical stability to 7kg/cm squared (7 bar/100psi), stability at a wide pH range (2-12), clean in place with 0.5M NaOH, and autoclavable. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects.
TOYOPEARL® HIC resins for hydrophobic interaction chromatography offer these advantages:
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.
- Strong affinity for water-soluble proteins
- High recovery of mass and activity
- High sample capacity: up to 2-4 times that of gel media
- Fluctuating salt concentrations will not change bed volume
- Mechanical stability to 7kg/cm2 (7 bar/100psi)
- Stable at wide pH range: 2-12
- Clean in place with 0.5M NaOH
- Autoclavable
TOYOPEARL media offer high yield recovery of proteins, using various aqueous eluants. The exclusion limit of 5x106 Da and large pore size, 1000Å, enables these packings to separate very large proteins by a hydrophobic interaction mechanism, without size exclusion effects. Packed as 100mL bottle.
Specifications
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Physical form
Shipped in 20% (v/v) ethanol.
Other Notes
Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in interresinte purification and polishing steps
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk_germany
WGK 1
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