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BIOshell Glycan (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

material

stainless steel hardware (column)

Quality Level

100

agency

suitable for USP L86

description

shell thickness (0.5 μm)
solid core (1.7 μm)

product line

BIOshell

feature

endcapped: no

analyte chemical class(es)

glycans

manufacturer/tradename

BIOshell

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 2.1 mm

surface area

135 m2/g

matrix

spherical silica particle platform
superficially porous particle

matrix active group

hydroxy, penta- phase

particle size

2.7 μm

pore size

90 Å

operating pH range

2-9

separation technique

hydrophilic interaction (HILIC)

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General description

BIOshell Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. The Fused-Core columns provide improved resolution when compared to conventional LC methods thus allowing more reliable quantitation and confident identification of glycans.

View the BIOshell Glycan HPLC Columns Flyer

Application

BIOshell Glycan HPLC Columns are high-performance liquid chromatography columns with Fused-Core particle design. Their column chemistry is suitable for hydrophilic interactive liquid chromatography (HILIC) analysis of biomolecules like oligosaccharides.

Features and Benefits

BIOshell Glycan HPLC Columns are compatible both with UHPLC and HPLC systems. They are specifically engineered for the fast and high-resolution seperation of biomolecules. These columns operate at high flow rates and have 40% higher efficiency than fully porous particles of the same size. They are stable at high temperatures, which increases the throughput and enhances the peak shape.

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Legal Information

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Certificates of Analysis (COA)

Lot/Batch Number
USJJL001326
USJJL001327
USJJL001321
USJJL001320
USJJL001319

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Articles

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

Rapid Glycan Prep & Analysis by LC-MS Workflow

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

Quality Attribute Determination of Monoclonal Antibodies

Workflows for monoclonal antibody adalimumab characterization ensure drug safety and efficacy through critical quality attribute analysis.

Protocols

Released N-Linked Glycan Analysis Workflow of Adalimumab

A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.

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HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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